RHMC - Partial Chromosome Paints (PCPs)

PCP (Partial Chromosome Paints) derived from:
Somatic cell hybrids (this page)	Microdissection	Examples of their use

PCP libraries, recognizing a definite region of a chromosome, are derived (via Alu-PCR) from somatic cell hybrids retaining fragments of human chromosomes. We have characterized about 900 of such fragments both from normal and radiation hybrids.

DNA samples (3-5ug) from these hybrids are available for free to researchers of non-profit Institutions, for cytogenetic research only (50-100ng of DNA are usually used for a 50ul PCR amplification; see Protocols).

PCPs specific for an entire arm (p or q)

PCP diagrams by chromosome
Chr. 1	Chr. 2	Chr. 3	Chr. 4	Chr. 5	Chr. 6
Chr. 7	Chr. 8	Chr. 9	Chr.10	Chr. 11	Chr. 12
Chr. 13	Chr. 14	Chr. 15	Chr. 16	Chr. 17	Chr. 18
Chr. 19	Chr. 20	Chr. 21	Chr. 22	Chr. X	Chr. Y
PCPs by code number order

The PCPs for each chromosome are grouped in two or three categories. The RHU group indicates hybrids retaining fragment(s) from a single chromosome. In the remaining groups the fragment of interest is not present as the only human contribution: RH are radiation fragments obtained from hybrids retaining more than one chromosome; HY indicates fragments from normal hybrids. Each PCP is represented by a solid bar specifying its extension and subchromosomal location. The bar is flanked by the code number, so that you can open and see the corresponding images.

*Alu PCR products produce an R-banding (you should keep in mind in evaluating the signals).

Note:only strong signals have been included in the diagrams. Some faint signals have been omitted also in the merged images. This just means that they are not appropriate as PCP.
Some chromosomes (chr.19 in particular) are Alu rich. In FISH experiments in which the COT1 DNA did not work properly, they appear quite strong, even though they are not present in the hybrid.

The following hybrids have been obtained from the NIGMS Human Genetic Mutant Cell Repository:

NIGMS code	code	NIGMS code	code	NIGMS code	code
GM10501 GM10662A GM10664 GM10890 GM11099 GM10663 GM10927B GM11418 GM07299A3 GM07299A7 GM10156C	002 003 004 005 006 108 150 151 157 158 200	GM10253A GM10411A GM10449A GM10478B GM10479A GM10498A GM10611A GM06317A GM10629A GM10662A GM10663	154 159 195 155 198 197 152 153 156 003 108	GM10664 GM10890 GM10927B GM11010A GM11099 GM11139 GM11418 GM13139 GM13140 . .	004 005 150 196 006 160 151 199 194 . .

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