PCPs (Partial Chromosome Paints) from microdissected chromosomes

The microdissected material has been first DOP-amplified for 3 round of amplification and it seems to work fine. We are performing additional rounds of amplification.

chromosome #	chromosome #	chromosome #
chrom. # 1p  chrom. # 1q  chrom. # 2p  chrom. # 2q  chrom. # 3p  chrom. # 3q  chrom. # 4p  chrom. # 4q  chrom. # 5p  chrom. # 5q  chrom. # 6p  chrom. # 6q  chrom. # 7p  chrom. # 7q  chrom. # 8p  chrom. # 8q	chrom. # 9p  chrom. # 9q  chrom. # 10p  chrom. # 10q  chrom. # 11p  chrom. # 11q  chrom. # 12p  chrom. # 12q  chrom. # 13p  chrom. # 13q  chrom. # 14p  chrom. # 14q  chrom. # 15p  chrom. # 15q  chrom. # 16p  chrom. # 16q	chrom. # 17p  chrom. # 17q  chrom. # 18p  chrom. # 18q  chrom. # 19p  chrom. # 19q  chrom. # 20p  chrom. # 20q  chrom. # 21p  chrom. # 21q  chrom. # 22p  chrom. # 22q  chrom. # Xp  chrom. # Xq

DOP - PCR

Primers: CCGACTCGAGNNNNNNATGTGG

PCR

1	95 C for 3'
2	95 C for 1'
3	30 C for 1'30"
4	0.2 C / s to 72 C
5	72 C for 3:00
6	go to step 2, 10 times
7	94 C for 1'
8	62 C for 1'
9	72 C for 3:00
10	72 Cfor 1' + 1" / cycle
11	go to step 7, 30 times
12	72 C for 10'