ABL/BCR translocation (9/22)

  The goal is the cloning of appropriate genomic probes from ABL and BCR loci wich, alone or in combination, can detect the splitting and/or the moving of the gene(s) under study. Their use in FISH experiments can be designed according to different strategies, as shown in the following drawings:
 Drawings  Real examples

 In our hands, the best combination of probes detecting the splitting of ABL and the colocalization of ABL/BCR on Ph chromosome is:

[dJ835J22 (ABL) + dJ1132H12 (ABL)] cohybridized with bc72M14 (BCR)*
9q34.12
chr9:130,740,224-130,792,614
chr9:130,574,219-130,695,829
chr9:130,644,636-130,813,821

ABL

  PAC  position (primers)

 FISH in

comments
1132H12

 Intron 1

primers 1

centromeric

CML t(9;22) case 2

 remains on der(9)

CML t(9;22) case 3 

 remains on der(9)

CML t(9;22) case 5

remains on der(9)

 CML t(9;22) case 6

remains on der(9) 

 CML t(9;22) case 7

 remains on der(9)

 CML t(9;22) case 4

== splits ==

CML t(9;22) case 1

  moves to der(22)
913J14

 Intron 1

primers 2

CML t(9;22) case 1

 moves to der(22)
888H11

Intron 1

primers 3

telomeric

 

CML t(9;22) case 1

 moves to der(22)
835J22

Exon 11

same primers

 CML t(9;22) case1

 moves to der(22)
1146L15

 CML t(9;22) case 1

  moves to der(22)
  Note that the most breakpoints on ABL are scattered over a large intron
  Details of all ABL probes with images on normal metaphases, primers used...

BCR

 BAC

 position

 FISH in

 comments
bC72M14

chr22:21,713,134-21,899,302

 CML t(9;22) case1

remains on der(22)
bK217D6    

CML, t(9;22)

  
bK52C3  

 CML, t(9;22)

 
 Additional probes and details of all BCR probes with images on normal metaphases, primers used...

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