PhD program in
Genetics and Molecular and Structural Evolution  
Coordinated by Prof. Mariano Rocchi

  Primate evolution; evolutionary centromere repositioning...

  M.Rocchi, N.Archidiacono, M.Ventura

The group has defined the chromosomal synteny arrangement of several primate species, using molecular cytogenetic techniques, BAC clones in particular.
The detailed comparison of primates' karyotypes allowed the reconstruction of the evolutionay history of all human chromosomes.

In some cases these works have been crucial in the correct sequence assembly. The following web pages display in a user friendly, figurative way, the chromosomal organization of macaque, orangutan, Hoolock leuconedys (gibbon), Nomascus leucogenys (gibbon), Hylobates lar (gibbon), Callitrix jacchus (marmoset).

Published papers (selection) (click to download the pdf)

Rocchi M, Stanyon R, Archidiacono N: Evolutionary new centromeres in primates. Prog Mol Subcell Biol 48:103-152 (2009)

Wade CM, Giulotto E, Sigurdsson S, Zoli M, Gnerre S, Imsland F, Lear TL, Adelson DL, Bailey E, Bellone RR, Blocker H, Distl O, Edgar RC, Garber M, Leeb T, et al.: Genome sequence, comparative analysis, and population genetics of the domestic horse. Science 326:865-867 (2009)

Carbone L, D'Addabbo P, Cardone MF, Teti MG, Misceo D, Vessere GM, de Jong PJ, Rocchi M: A satellite-like sequence, representing a "clone gap" in the human genome, was likely involved in the seeding of a novel centromere in macaque. Chromosoma 118:269-277 (2009)

Capozzi O, Purgato S, D'Addabbo P, Archidiacono N, Battaglia P, Baroncini A, Capucci A, Stanyon R, Della Valle G, Rocchi M: Evolutionary descent of a human chromosome 6 neocentromere: a jump back to 17 million years ago. Genome Res 19:778-784 (2009)

Misceo D, Capozzi O, Roberto R, Dell'Oglio MP, Rocchi M, Stanyon R, Archidiacono N: Tracking the complex flow of chromosome rearrangements from the Hominoidea ancestor to extant Hylobates and Nomascus gibbons by high-resolution synteny mapping. Genome Res 18:1530-1537 (2008)

Ventura M, Antonacci F, Cardone MF, Stanyon R, D'Addabbo P, Cellamare A, Sprague LJ, Eichler EE, Archidiacono N, Rocchi M: Evolutionary formation of new centromeres in macaque. Science 316:243-246 (2007)

Roberto R, Capozzi O, Wilson RK, Mardis ER, Lomiento M, Tuzun E, Cheng Z, Mootnick AR, Archidiacono N, Rocchi M, Eichler EE: Molecular refinement of gibbon genome rearrangement. Genome Res 17:249-257 (2007)

Cardone MF, Alonso A, Pazienza M, Ventura M, Montemurro G, Carbone L, de Jong PJ, Stanyon R, D'Addabbo P, Archidiacono N, She X, Eichler EE, Warburton PE, Rocchi M: Independent centromere formation in a capricious, gene-free domain of chromosome 13q21 in Old World monkeys and pigs. Genome Biol 7:R91 (2006)

Newman TL, Tuzun E, Morrison VA, Hayden KE, Ventura M, McGrath SD, Rocchi M, Eichler EE: A genome-wide survey of structural variation between human and chimpanzee. Genome Res 15:1344-1356 (2005)

Cheng Z, Ventura M, She X, Khaitovich P, Graves T, Osoegawa K, Church D, DeJong P, Wilson RK, Paabo S, Rocchi M, Eichler EE: A genome-wide comparison of recent chimpanzee and human segmental duplications. Nature 437:88-93 (2005)

Consortium, ..., Rocchi M, Archidiacono N, Ventura M, ... Initial sequence of the chimpanzee genome and comparison with the human genome. Nature 437:69-87 (2005)

Ventura M, Weigl S, Carbone L, Cardone MF, Misceo D, Teti M, D'Addabbo P, Wandall A, Bjorck E, de Jong P, She X, Eichler EE, Archidiacono N, Rocchi M: Recurrent sites for new centromere seeding. Genome Res 14:1696-1703 (2004)

Ventura M, Mudge JM, Palumbo V, Burn S, Blennow E, Pierluigi M, Giorda R, Zuffardi O, Archidiacono N, Jackson MS, Rocchi M: Neocentromeres in 15q24-26 map to duplicons which flanked an ancestral centromere in 15q25. Genome Res 13:2059ñ2068 (2003)

Eder V, Ventura M, Ianigro M, Teti M, Rocchi M, Archidiacono N: Chromosome 6 phylogeny in primates and centromere repositioning. Mol Biol Evol 20:1506-1512 (2003)

Johnson ME, Viggiano L, Bailey JA, Abdul-Rauf M, Goodwin G, Rocchi M, Eichler EE: Positive selection of a gene family during the emergence of humans and African apes. Nature 413:514-519 (2001)

Jackson MS, Rocchi M, Thompson G, Hearn T, Crosier M, Guy J, Kirk D, Mulligan L, Ricco A, Piccininni S, Marzella R, Viggiano L, Archidiacono N: Sequences flanking the centromere of human chromosome 10 are a complex patchwork of arm-specific sequences, stable duplications and unstable sequences with homologies to telomeric and other centromeric locations. Hum Mol Genet 8:205-215 (1999)

Montefalcone G, Tempesta S, Rocchi M, Archidiacono N: Centromere repositioning. Genome Res 9:1184-8 (1999)