Molecular refinement of gibbon genome rearrangements

Roberta Roberto,1* Oronzo Capozzi,1* Richard K. Wilson,2 Elaine R. Mardis,2 Mariana Lomiento,1 Eray Tuzun,3 Ze Cheng,3 Alan R. Mootnick,4 Nicoletta Archidiacono,1 Mariano Rocchi,1,6 and Evan E. Eichler3,5,6
1Department of Genetics and Microbiology, University of Bari, 70127 Bari, Italy; 2Washington University Genome Sequencing Center, Washington University School of Medicine, St. Louis, MO 63108; 3Department of Genome Sciences, University of Washington, Seattle, Washington 98195; 4Gibbon Conservation Center, Santa Clarita, CA, 91380; 5Howard Hughes Medical Institute, Seattle, Washington 98195

Publisced in Genome Research, 17: 249-257 (2007)

Nomascus leucogenys, NLE (white cheeked crested gibbon)

NLE karyotype (DAPI)       NLE karyogram          Hominoidea ancestral karyotype

NLE synteny blocks arrangement (summary)         HSA synteny blocks arrangement (summary)

NLE chromosome organization with respect to human (only relevant clones are reported)
2
3
4
5
6
7
8
9
 21 22b 23 24 25 X

Human chromosomes contributing to each NLE chromosome
NLE
1b
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
 21 22b 23 24 25
HSA 6
9
14		 5
16		 6
10		 3
8
11
18		 1
13		 5
15		 4
5
22		 3
6
8
9
12		 1
4
10
13		 12
19		 3
7
12
20		 1 7
20		 2
17		 11 8 2
6
7
19		 4
5
6
10
16		 2
17		 2
4		 3
11		 2
6
14
 12 1 21

NLE chromosomes contributing to each human chromosome
HSA
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
NLE 5
9
12
24		 14
17
19
20
22b		 4
8
11
21		 7
9
18
20		 2
6
7
18		 1b
3
8
17
18
22		 11
13
17		 4
8
16		 1b
8		 3
9
18		 4
15
21		 8
10
11
23		 5
9		 1b
22b		 6 2
18
 14
19		 4 10
17		 11
13		 25 7


FISH examples of splitting clones (see also NLE chromosome-specific pages)

Split in HSA (NLE probes) Split in NLE (HSA probes)
clone
NLE
HSA
clone
HSA
NLE
6b/9b2
HSA1
NLE 5/12
9b1/9b4
HSA1
NLE 9/12
5c1/16b1
HSA2
NLE 17/20
5c2/16b1
HSA2
NLE 2/2
18a1/18a2
HSA6
NLE 17/22
5b/16a
HSA7
NLE 17/20
1b1/1b3
HSA8
NLE 16/16
7c1/7c2
HSA9
NLE 1b/8
1c1/1c3
HSA9
 NLE 1b/8
2d/7a1
HSA10
NLE 3/18
2b2/17b4
HSA16
 NLE 2/2
14a1/14a2
HSA20
NLE 11/13
Xa1/Xa2
HSA20
NLE 11/13


Examples of NLE probes yielding duplicated FISH signals

Clone
NLE
HSA
 comment
CH271-71L9 Signals, in NLE, in telomeric and other heterochromatic regions
CH271-139C8
CH271-156E13 Multiple signals
CH271-174G4 Multiple signals
CH271-337F19 Duplication
CH271-327M6 Multiple signals
CH271-202C14 Morpheus like
CH271-342G19 Telomeric and interstitial heterochromatin
CH271-118P10 Single s. in NLE; m.s., telomeric or pericentromeric, in HSA
CH271-330L11 m. s. in NLE; faint signals in sone centromeres of HSA
CH271-146B14 Multiple centromeric signals