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Immunoglobulin genes IgK at 2p11.2, in normal cells Atlas IgK |
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| IgK at UCSC | ||
| Primers (5' -> 3') |
Clone |
Comment |
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dJ1117G4 | see below |
Ends of dJ1117G4:
GATCTTCACGCAGGCCTCATGTTAGAGACTGCTTGGCACCATTTTGGCTCCTGCACACCTGTAAGTGTCC
CATCTACCCACCTGCCCTATGTCAGATGGTCCAGGAAATAAGGCCTTTGGCTAAATAGTCCATTTGAAGT
CCCCCATAGCACACCTGACTACATCCTCATTGATCTTTCTCTTAGTCATTTCTCCTCTAATGACATTTTA
TTTATCCATCAGCCATTTTATCTTATTTTCTGTCCTGATATATGTGTTTGCTTTGCAGTTTTTTCTTTGG
GTCCCTACTAATTATGTTTGTGCAATTGTTTAAGGCAGGACACTTGGATGTAAGAATTCTCCTGTTCTGT
TGACTCTAAGAAGCCAGAGTCACATTGTTCTATGGCCCAACCAGGCCTTTGGGGCTCATTGTTGGCCACC
CCACTGAGGCTNCAGGATTTTCTG (do not contain single copy sequences)
AZ757842:
AGGATCTGGATAACCCGAAGCAAGAATTTCTCTGTCAGTTTTCTCATCTA
GTATAATGGTAGAGAGTGGGGGTGTTGAAGTAGAAAAATCCTTTGATTAA
TATTTATTCTAATCCTTGACTAGGTGCTCAGTGTTAGTCCAGGGAACTCT
ATCTTATTTNTTNNTGGGCCAGCTTAATGTCAAGATCCAAAACTCTTGTG
TAAAGCTTGGGACCATTTTCAAGGATACATTTAGTAGTTTACAATAATCT
AGATAACACTGACTGATAGAAATATGCAGGAAAT
BlastN of this sequence:
>AC023416
(clone RP11-601N4, 176555 bp, complete sequence)
Minus Strand HSPs:
Query: 284 ATTTCCTGCATATTTCTATCAGTCAGTGTTATCTAGATTATTGTAAACTACTAAATGTAT
225
ATTTCCTGCATATTTCTATCAGTCAGTGTTATCTAGATTATTGTAAACTACTAAATGTAT
Sbjct: 3265 ATTTCCTGCATATTTCTATCAGTCAGTGTTATCTAGATTATTGTAAACTACTAAATGTAT
3324
Query: 224 CCTTGAAAATGGTCCCAAGCTTTACACAAGAGTTTTGGATCTTGACATTAAGCTGGCCCA
165
CCTTGAAAATGGTCCCAAGCTTTACACAAGAGTTTTGGATCTTGACATTAAGCTGGCCCA
Sbjct: 3325 CCTTGAAAATGGTCCCAAGCTTTACACAAGAGTTTTGGATCTTGACATTAAGCTGGCCCA
3384
Query: 164 NNAANAAATAAGATAGAGTTCCCTGGACTAACACTGAGCACCTAGTCAAGGATTAGAATA
105
AA
AAATAAGATAGAGTTCCCTGGACTAACACTGAGCACCTAGTCAAGGATTAGAATA
Sbjct: 3385 TGAAGAAATAAGATAGAGTTCCCTGGACTAACACTGAGCACCTAGTCAAGGATTAGAATA
3444
Query: 104 AATATTAATCAAAGGATTTTTCTACTTCAACACCCCCACTCTCTACCATTATACTAGATG
45
AATATTAATCAAAGGATTTTTCTACTTCAACACCCCCACTCTC
ACCATTATAC AGATG
Sbjct: 3445 AATATTAATCAAAGGATTTTTCTACTTCAACACCCCCACTCTC-ACCATTATAC-AGATG
3502
Query: 44 AGAAAACTGACAGAGAAATTCTTGCTTCGGGTTATCCAGATCCT
1
AGAAAACTGACAGAGAAATTCTTGCTTCGGGTTATCCAGATCCT
Sbjct: 3503 AGAAAACTGACAGAGAAATTCTTGCTTCGGGTTATCCAGATCCT 3546
Conclusions: the dJ1117G4 overlaps for few kb with RP11-601N4, consequently it could overlap for most part of RP11450E9.
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