SUPPLEMENTAL FILE SF3
Chromosomal flow changes from Hylobatidae ancestor to NLE
(Refer to Supplemental table ST3, ST4)
(click to enlarge each Figure)
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SUPPLEMENTAL FILE SF3
Chromosomal flow changes from Hylobatidae ancestor to NLE
(Refer to Supplemental table ST3, ST4)
(click to enlarge each Figure)
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The 8 Slides composing the Supplemental File 3 report the flow of chromosomal changes from Hylobatidae ancestor (HyA, as shown in Figure 4 and in supplemental table ST4) to Nomascus leucogenys (Supplemental Fig1 and Supplemental table ST3). Ideograms in dotted rectangles indicate intermediate chromosomal forms. Ideograms of final NLE chromosomes are in red rectangles. In nature, there are two forms of NLE karyotype. The second derivative form differs for a translocation involving chromosomes NLE1 and NLE22. The NLE cell line we analyzed has this translocation. The derivative chromosomes are indicated as NLE1b and NLE22b (original forms: NLE1a and NLE22a) (Koehler 1995). The two karyotypes maybe specific to two distinct subpopulations because individuals heterozygous for the two forms have never been reported in the wild.
The symbol Green hatched boxes include chromosomes involved in the rearrangement; blue dotted boxes contain intermediate chromosomes, while red boxes indicate the final chromosomal form. The numbers on the right of the final Hylobatidae ancestor chromosome (HyA) represent arbitrarily chosen points useful in indicating its composition, as reported in ST4. Usually they correspond to a BAC. When found at the borders of the synteny blocks they identify two BACs facing each other following a rearrangement. :: (double dots) indicate breakage and reunion. NEC stands for new evolutionary centromere. 
Inactivated centromere.
Proportions among segments composing a chromosomes is only approximate because forr graphic reasons, to better show the composition of small chromosomes, the proportions among chromosomes were often altered.
References:
- Koehler, U., Bigoni, F., Wienberg, J., and Stanyon, R. 1995.
Genomic reorganization in the concolor gibbon (Hylobates concolor) revealed by chromosome painting. Genomics 30: 287-292.
