SUPPLEMENTAL TABLES:
(zipped Excel or Word files; click to download)
Supplemental Table ST1
The Table shows the organization of human chromosomes with respect to HLA (column E) and NLE (Column F). Yellow rows indicate that a specific BAC, or the region defined by two closely mapping or overlapping BACs, encompass a break in HLA, in NLE, or in both. In the latter case the break occurred in the Hylobatidae Ancestor (HyA), as reported in the column G. Multiple signals due to crosshybridizations are reported in pink.
Supplemental Table ST2
The Table summarizes the HLA chromosome organization with respect to Hylobatidae Ancestral Karyotype (HyAK, reported in Supplemental Table S4). The numbers reported in column A represent arbitrarily chosen landmarks useful in defining the chromosome composition. Most of them correspond to a specific BAC. Other landmarks are generated by regions brought face to face as a consequence of a rearrangement. In the latter case, the landmark corresponds to the two BACs that were joined following the rearrangement. Column B also reports some NLE BAC clones belonging to the NLE BAC library (in blue cells), whose mapping is supported by BAC-end sequencing and FISH analysis. Cells in light green indicate ancestral syntenies in Hylobatidae and/or Hominoidea. Orange cells indicate newly generated syntenic regions. Cells in yellow contain a splitting BAC or BACs that flank a splitting region. Multiple signals due to crosshybridizations are reported in pink.
Supplemental Table ST3
Reconstruction of NLE chromosomes with respect to the Hylobatidae Ancestor (Supplemental Table S4). The numbers reported in column A represent arbitrarily chosen landmarks useful in defining the chromosome composition. Most of them correspond to a specific BAC. Other landmarks are generated by regions brought face to face as a consequence of a rearrangement. In the latter case the landmark corresponds to the two BACs that were joined following the rearrangement. Column B also reports some NLE BAC clones belonging to the NLE BAC library (in blue cells), whose mapping is supported by BAC-end sequencing and FISH analysis. Cells in light green indicate ancestral syntenies in Hylobatidae and/or Hominoidea. Orange cells indicate newly generated syntenic regions. Cells in yellow contain a splitting BAC or BACs that flank a splitting region. Multiple signals due to crosshybridizations are reported in pink.
Supplemental Table ST4
Reconstruction of Hylobatidaes Ancestral karyotype. The numbers reported in column A represent arbitrarily chosen landmarks useful in defining the chromosome composition. Most of them correspond to a specific BAC. Other landmarks are generated by regions brought face to face as a consequence of a rearrangement. In the latter case the landmark corresponds to the two BACs that were joined following the rearrangement. Column B also reports some NLE BAC clones belonging to the NLE BAC library (in blue cells), whose mapping is supported by BAC-end sequencing and FISH analysis. Cells in light green indicate ancestral syntenies in Hylobatidae and/or Hominoidea. Orange cells indicate newly generated syntenic regions. Cells in yellow contain a splitting BAC or BACs that flank a splitting region. Multiple signals due to crosshybridizations are reported in pink.
Supplemental Table ST5
Reconstruction of Hominoidea karyotype, according to the data reported by Stanyon et al. (2008), with respect to the Hylobatidae Ancestral Karyotype (column A, HyAK, according to the Supplemental Table S4). The numbers reported in column A represent arbitrarily chosen landmarks useful in defining the chromosome composition. Most of them correspond to a specific BAC. Other landmarks are generated by regions brought face to face as a consequence of a rearrangement. In the latter case the landmark corresponds to the two BACs that were joined following the rearrangement. Column B also reports some NLE BAC clones belonging to the NLE BAC library (in blue cells), whose mapping is supported by BAC-end sequencing and FISH analysis. Cells in light green indicate ancestral syntenies in Hylobatidae and/or Hominoidea. Orange cells indicate newly generated syntenic regions. Cells in yellow contain a splitting BAC or BACs that flank a splitting region. Multiple signals due to crosshybridizations are reported in pink.
Supplemental Table ST6
The Table lists the BACs that identify breakpoint domains, in the Hylobatidae ancestor, which were reused in independent evolutionary rearrangements in other primate species. In case 3, two BACs, RP11-467J24 and RP11-715P22, that identify break domains in the Hylobatidae ancestor and in Trachypithecus cristatus (silvered leaf-monkey, Old World monkey, chromosome 9/16 translocation) respectively, are ~550kb apart. The last domain was also used as an evolutionary new centromere seeding-point (NEC) in the HLA/NLE ancestor and in humans.