The position of the clones on the human sequence is derived from the UCSC database.

Generation of smaller probes using long-PCR. Oligos from the sequence of a specific region can be designed to amplify DNA stretches of appropriate length by long-PCR experiments suitable for FISH experiments multiple. The use of repeat-masked sequences can help in avoiding long repeats that would poorly contributed to the FISH signal. The efficiency and specificity of PCR amplification can be improved by using the appropriate BAC/PAC probe as template, in alternative to the total human DNA. Relatively small long-PCR products (few kb) can be pooled for this purpose. In our hands cloned PCR products are more efficient in FISH experiments than amplified products.