Most of the clones belong to the BAC or PAC libraries produced by P. de Jong (BAC/PAC libraries).

Codes: sometimes the prefix used for BACs and PACs (dJ..., bA...) is the one used at the Sanger Centre: see specific page.
m.s. = multiple site (two or more sites detected by the clone).

The position of the clones on the human sequence is derived from the UCSC database.

Generation of smaller probes using long-PCR. Oligos from the sequence of a specific region can be designed to amplify DNA stretches of appropriate length by long-PCR experiments suitable for FISH experiments multiple. The use of repeat-masked sequences can help in avoiding long repeats that would poorly contributed to the FISH signal. The efficiency and specificity of PCR amplification can be improved by using the appropriate BAC/PAC probe as template, in alternative to the total human DNA. Relatively small long-PCR products (few kb) can be pooled for this purpose. In our hands cloned PCR products are more efficient in FISH experiments than amplified products.

We send our reagents in this form:

BAC / PAC / YAC / plasmids stab (bacteria containing the probe, in agar). To be quick, we just prepare the stab and put it on the mail. You may need to keep it at 37C for some hours. In addition, you are supposed to grow the bacteria, extract, label DNA etc. to be stored in the refrigerator (not in the freezer), or at room temperature.
PCP and WCP (somatic cell hybrids) DNA; to be amplified by Alu-PCR to be stored in the refrigerator
WCP (flow-sorted chromosomes) DOP-amplified products; to be amplified again with DOP-PCR to be stored in the refrigerator

PAC, BAC, YAC library information  (resistance...)

NOTE: we are moving the link sto the UCSC (University California Santa ruz)