Mouse PAC clones


 The project is aimed at characterizing a panel of PAC clones, at least one for each mouse chromosome, to be used as tag markers in mapping experiments. Mouse PAC clones (RPCI-21 library) have been hybridized to a peculiar 8I1-3 mouse cell line (QM-karyotype; DAPI karyotype) with multiple Robertsonian translocations (Mus musculus domesticus, wmp/wmp) which allow an easy identification of mouse chromosomes (generous gift of Dr. Hameister; Cyt.Cell Genet. 71:37- 40, 1995). The RPCI-21 PAC library segment consisting of 132.000 clones constructed from a female 129/SvEvTACfBr. The average insert size is 150 Kb, corresponding to 6 genome equivalents.

 Chrom.*  clone  mapping  cohybridizations
  1 (11)  478F21 #  1A  1-11 (478F21+476M4)
 480G1  1H  
  2 (16)  344M24 &#  2H  2-16 (344M24+428H10)
 379H5  2H  
 473L8 #  2H / XA  2-16 (473L8+428H10)
  3 (12)  428M21 #  3F  3-12 (428M21+426P10)
 428C4  #  3G-H  3-12 (428C4+426P10)
  4 (6)  372I17 &  4B  4-6 (669N24+372I17)
 652E10 &  4B / XA2  
  5 (14)  478L2 #  5F  5-14 [478L2+(478E2+473J24)]
 426A18 #  5G  
  6 (4)  669N24 #  6C  6-4 (669N24+372I17)
  7 (18)  353C3 #  7B  7-18 (497L11+353C3)
 430A21  7F  
  8 (9)  344N12 &#    8D  8-9 (344N12+480M9)
  9 (8)  480M9  #  9D  9-8 (344N12+480M9)
 10 (17)  367J7 #  10A  10-17 (367J7+669I17)
 523I19 #  10D  
 11 (1)  476M4 #  11C  11-1 (478F21+476M4)
 12 (3)  426P10  12E  12-3 (428M21+426P10)
 12-3 (428C4+426P10)
 13 (15)  669F3 #  13A  13-15 (669F3 + 561P12)
 14 (5)  478E2 #  14A  14-5 [(478E2+473J24)+478L2]
 473J24 #  14C
 445I19 #  14E  
 15 (13)  561P12 #  15E  15-13 (669F3 + 561P12)
 16 (2)  428H10 #  16A  16-2 (473L8+428H10)
 16-2 (
344M24+428H10)
 669H10  16B  
 17 (10)  669I17 &  17E  17-10 (367J7+669I17)
 18 (7)  497L11 &    18-7 (497L11+353C3)
 19  607E15 &#  19B  
  X  480L23 #  XD  
 473L8 #  XA / 2H  

* in patenthesis: the fusion partner
#
= CONFIRMED DATA (the probe maps on a marker chromosome or it has been cohybridized with a probe mapping on the partner chromosome...).
&= PAC derived by screening the library with an STS whose mapping was known (MIT database)
 Stabs from these PACs can be obtained from the YAC screening Center, Milan ,
 e-mail toniolo.daniela@hsr.it; tl. +39-02-2643.4775 ; fax +39-02-2643.4855)

 Useful information on mouse genome

 
Mouse G-banding ideograms

QM-karyotype   --   DAPI karyotype

An animated picture (Molecular mechanism of Robertsonian fusion)

 Mouse/human homologies (The Jackson Laboratory)

Oxford grid
Comparative maps



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