HyA6, HyA19:
We agree with Muller et al. (2003) that a centric fission generated two chromosomes, HyA6 and HyA19. One breakpoint was inside marker K. The second break was centromeric/pericentromeric. The erratic position of the markers F, G, and H in human, in HLA6 and HLA20 (corresponding to HyA6 and HyA19) can very likely be attributed to the plasticity of pericentromeric regions that, in humans, is higher than depicted by the hg18 assembly. Clone G (RP11-484F20), indeed, gave signals on both sides of the human centromere. The duplication was not reported in the hg18 assembly.
The marker 13D splits in both HLA and NLE, and therefore represents one of the two breaks of the inversion. The only open question is the position of the centromere in HyA8. In other words, if the inversion was paracentric or pericentric. The inversion appears pericentric in NLE (Roberto et al. 2007), while paracentric in HLA (present work) and, very likely, it was also paracentric in Hoolock: in HHO, the short arm of chromosome 2 was hybridized by NLE9 and NLE5, which derived from HyA8. The position of the two paint hybridizations suggests that the ancestral form, HyA8, was acrocentric. The present interpretation was favored because it is the most parsimonious.
Muller, S., Hollatz, M., and Wienberg, J. 2003. Chromosomal phylogeny and evolution of gibbons (Hylobatidae). Hum. Genet. 113: 493-501.
Nie, W., Rens, W., Wang, J., and Yang, F. 2001. Conserved chromosome segments in Hylobates hoolock revealed by human and H. leucogenys paint probes. Cytogenet. Cell Genet. 92: 248-253.