Primer3 Output
PRIMER PICKING RESULTS FOR gi|169790829|ref|NM_001754.4| Homo sapiens runt-related transcription factor 1 (RUNX1), transcript variant 1, mRNA
No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 4412 20 60.01 55.00 2.00 0.00 CATTTCCCTCCCTCTCTTCC
RIGHT PRIMER 4653 20 59.99 50.00 3.00 2.00 TGGCAATGTCTCTGTTCTGC
SEQUENCE SIZE: 5967
INCLUDED REGION SIZE: 5967
PRODUCT SIZE: 242, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
1 CTTTGGGCCTCATAAACAACCACAGAACCACAAGTTGGGTAGCCTGGCAGTGTCAGAAGT
61 CTGAACCCAGCATAGTGGTCAGCAGGCAGGACGAATCACACTGAATGCAAACCACAGGGT
121 TTCGCAGCGTGGTAAAAGAAATCATTGAGTCCCCCGCCTTCAGAAGAGGGTGCATTTTCA
181 GGAGGAAGCGATGGCTTCAGACAGCATATTTGAGTCATTTCCTTCGTACCCACAGTGCTT
241 CATGAGAGAATGCATACTTGGAATGAATCCTTCTAGAGACGTCCACGATGCCAGCACGAG
301 CCGCCGCTTCACGCCGCCTTCCACCGCGCTGAGCCCAGGCAAGATGAGCGAGGCGTTGCC
361 GCTGGGCGCCCCGGACGCCGGCGCTGCCCTGGCCGGCAAGCTGAGGAGCGGCGACCGCAG
421 CATGGTGGAGGTGCTGGCCGACCACCCGGGCGAGCTGGTGCGCACCGACAGCCCCAACTT
481 CCTCTGCTCCGTGCTGCCTACGCACTGGCGCTGCAACAAGACCCTGCCCATCGCTTTCAA
541 GGTGGTGGCCCTAGGGGATGTTCCAGATGGCACTCTGGTCACTGTGATGGCTGGCAATGA
601 TGAAAACTACTCGGCTGAGCTGAGAAATGCTACCGCAGCCATGAAGAACCAGGTTGCAAG
661 ATTTAATGACCTCAGGTTTGTCGGTCGAAGTGGAAGAGGGAAAAGCTTCACTCTGACCAT
721 CACTGTCTTCACAAACCCACCGCAAGTCGCCACCTACCACAGAGCCATCAAAATCACAGT
781 GGATGGGCCCCGAGAACCTCGAAGACATCGGCAGAAACTAGATGATCAGACCAAGCCCGG
841 GAGCTTGTCCTTTTCCGAGCGGCTCAGTGAACTGGAGCAGCTGCGGCGCACAGCCATGAG
901 GGTCAGCCCACACCACCCAGCCCCCACGCCCAACCCTCGTGCCTCCCTGAACCACTCCAC
961 TGCCTTTAACCCTCAGCCTCAGAGTCAGATGCAGGATACAAGGCAGATCCAACCATCCCC
1021 ACCGTGGTCCTACGATCAGTCCTACCAATACCTGGGATCCATTGCCTCTCCTTCTGTGCA
1081 CCCAGCAACGCCCATTTCACCTGGACGTGCCAGCGGCATGACAACCCTCTCTGCAGAACT
1141 TTCCAGTCGACTCTCAACGGCACCCGACCTGACAGCGTTCAGCGACCCGCGCCAGTTCCC
1201 CGCGCTGCCCTCCATCTCCGACCCCCGCATGCACTATCCAGGCGCCTTCACCTACTCCCC
1261 GACGCCGGTCACCTCGGGCATCGGCATCGGCATGTCGGCCATGGGCTCGGCCACGCGCTA
1321 CCACACCTACCTGCCGCCGCCCTACCCCGGCTCGTCGCAAGCGCAGGGAGGCCCGTTCCA
1381 AGCCAGCTCGCCCTCCTACCACCTGTACTACGGCGCCTCGGCCGGCTCCTACCAGTTCTC
1441 CATGGTGGGCGGCGAGCGCTCGCCGCCGCGCATCCTGCCGCCCTGCACCAACGCCTCCAC
1501 CGGCTCCGCGCTGCTCAACCCCAGCCTCCCGAACCAGAGCGACGTGGTGGAGGCCGAGGG
1561 CAGCCACAGCAACTCCCCCACCAACATGGCGCCCTCCGCGCGCCTGGAGGAGGCCGTGTG
1621 GAGGCCCTACTGAGGCGCCAGGCCTGGCCCGGCTGGGCCCCGCGGGCCGCCGCCTTCGCC
1681 TCCGGGCGCGCGGGCCTCCTGTTCGCGACAAGCCCGCCGGGATCCCGGGCCCTGGGCCCG
1741 GCCACCGTCCTGGGGCCGAGGGCGCCCGACGGCCAGGATCTCGCTGTAGGTCAGGCCCGC
1801 GCAGCCTCCTGCGCCCAGAAGCCCACGCCGCCGCCGTCTGCTGGCGCCCCGGCCCTCGCG
1861 GAGGTGTCCGAGGCGACGCACCTCGAGGGTGTCCGCCGGCCCCAGCACCCAGGGGACGCG
1921 CTGGAAAGCAAACAGGAAGATTCCCGGAGGGAAACTGTGAATGCTTCTGATTTAGCAATG
1981 CTGTGAATAAAAAGAAAGATTTTATACCCTTGACTTAACTTTTTAACCAAGTTGTTTATT
2041 CCAAAGAGTGTGGAATTTTGGTTGGGGTGGGGGGAGAGGAGGGATGCAACTCGCCCTGTT
2101 TGGCATCTAATTCTTATTTTTAATTTTTCCGCACCTTATCAATTGCAAAATGCGTATTTG
2161 CATTTGGGTGGTTTTTATTTTTATATACGTTTATATAAATATATATAAATTGAGCTTGCT
2221 TCTTTCTTGCTTTGACCATGGAAAGAAATATGATTCCCTTTTCTTTAAGTTTTATTTAAC
2281 TTTTCTTTTGGACTTTTGGGTAGTTGTTTTTTTTTGTTTTGTTTTGTTTTTTTGAGAAAC
2341 AGCTACAGCTTTGGGTCATTTTTAACTACTGTATTCCCACAAGGAATCCCCAGATATTTA
2401 TGTATCTTGATGTTCAGACATTTATGTGTTGATAATTTTTTAATTATTTAAATGTACTTA
2461 TATTAAGAAAAATATCAAGTACTACATTTTCTTTTGTTCTTGATAGTAGCCAAAGTTAAA
2521 TGTATCACATTGAAGAAGGCTAGAAAAAAAGAATGAGTAATGTGATCGCTTGGTTATCCA
2581 GAAGTATTGTTTACATTAAACTCCCTTTCATGTTAATCAAACAAGTGAGTAGCTCACGCA
2641 GCAACGTTTTTAATAGGATTTTTAGACACTGAGGGTCACTCCAAGGATCAGAAGTATGGA
2701 ATTTTCTGCCAGGCTCAACAAGGGTCTCATATCTAACTTCCTCCTTAAAACAGAGAAGGT
2761 CAATCTAGTTCCAGAGGGTTGAGGCAGGTGCCAATAATTACATCTTTGGAGAGGATTTGA
2821 TTTCTGCCCAGGGATTTGCTCACCCCAAGGTCATCTGATAATTTCACAGATGCTGTGTAA
2881 CAGAACACAGCCAAAGTAAACTGTGTAGGGGAGCCACATTTACATAGGAACCAAATCAAT
2941 GAATTTAGGGGTTACGATTATAGCAATTTAAGGGCCCACCAGAAGCAGGCCTCGAGGAGT
3001 CAATTTGCCTCTGTGTGCCTCAGTGGAGACAAGTGGGAAAACATGGTCCCACCTGTGCGA
3061 GACCCCCTGTCCTGTGCTGCTCACTCAACAACATCTTTGTGTTGCTTTCACCAGGCTGAG
3121 ACCCTACCCTATGGGGTATATGGGCTTTTACCTGTGCACCAGTGTGACAGGAAAGATTCA
3181 TGTCACTACTGTCCGTGGCTACAATTCAAAGGTATCCAATGTCGCTGTAAATTTTATGGC
3241 ACTATTTTTATTGGAGGATTTGGTCAGAATGCAGTTGTTGTACAACTCATAAATACTAAC
3301 TGCTGATTTTGACACATGTGTGCTCCAAATGATCTGGTGGTTATTTAACGTACCTCTTAA
3361 AATTCGTTGAAACGATTTCAGGTCAACTCTGAAGAGTATTTGAAAGCAGGACTTCAGAAC
3421 AGTGTTTGATTTTTATTTTATAAATTTAAGCATTCAAATTAGGCAAATCTTTGGCTGCAG
3481 GCAGCAAAAACAGCTGGACTTATTTAAAACAACTTGTTTTTGAGTTTTCTTATATATATA
3541 TTGATTATTTGTTTTACACACATGCAGTAGCACTTTGGTAAGAGTTAAAGAGTAAAGCAG
3601 CTTATGTTGTCAGGTCGTTCTTATCTAGAGAAGAGCTATAGCAGATCTCGGACAAACTCA
3661 GAATATATTCACTTTCATTTTTGACAGGATTCCCTCCACAACTCAGTTTCATATATTATT
3721 CCGTATTACATTTTTGCAGCTAAATTACCATAAAATGTCAGCAAATGTAAAAATTTAATT
3781 TCTGAAAAGCACCATTAGCCCATTTCCCCCAAATTAAACGTAAATGTTTTTTTTCAGCAC
3841 ATGTTACCATGTCTGACCTGCAAAAATGCTGGAGAAAAATGAAGGAAAAAATTATGTTTT
3901 TCAGTTTAATTCTGTTAACTGAAGATATTCCAACTCAAAACCAGCCTCATGCTCTGATTA
3961 GATAATCTTTTACATTGAACCTTTACTCTCAAAGCCATGTGTGGAGGGGGCTTGTCACTA
4021 TTGTAGGCTCACTGGATTGGTCATTTAGAGTTTCACAGACTCTTACCAGCATATATAGTA
4081 TTTAATTGTTTCAAAAAAAATCAAACTGTAGTTGTTTTGGCGATAGGTCTCACGCAACAC
4141 ATTTTTGTATGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGAAAAATTGCATTCATTG
4201 ACTTCAGGTAGATTAAGGTATCTTTTTATTCATTGCCCTCAGGAAAGTTAAGGTATCAAT
4261 GAGACCCTTAAGCCAATCATGTAATAACTGCATGTGTCTGGTCCAGGAGAAGTATTGAAT
4321 AAGCCATTTCTACTGCTTACTCATGTCCCTATTTATGATTTCAACATGGATACATATTTC
4381 AGTTCTTTCTTTTTCTCACTATCTGAAAATACATTTCCCTCCCTCTCTTCCCCCCAATAT
>>>>>>>>>>>>>>>>>>>>
4441 CTCCCTTTTTTTCTCTCTTCCTCTATCTTCCAAACCCCACTTTCTCCCTCCTCCTTTTCC
4501 TGTGTTCTCTTAAGCAGATAGCACATACCCCCACCCAGTACCAAATTTCAGAACACAAGA
4561 AGGTCCAGTTCTTCCCCCTTCACATAAAGGAACATGGTTTGTCAGCCTTTCTCCTGTTTA
4621 TGGGTTTCTTCCAGCAGAACAGAGACATTGCCAACCATATTGGATCTGCTTGCTGTCCAA
<<<<<<<<<<<<<<<<<<<<
4681 ACCAGCAAACTTTCCTGGGCAAATCACAATCAGTGAGTAAATAGACAGCCTTTCTGCTGC
4741 CTTGGGTTTCTGTGCAGATAAACAGAAATGCTCTGATTAGAAAGGAAATGAATGGTTCCA
4801 CTCAAATGTCCTGCAATTTAGGATTGCAGATTTCTGCCTTGAAATACCTGTTTCTTTGGG
4861 ACATTCCGTCCTGATGATTTTTATTTTTGTTGGTTTTTATTTTTGGGGGGAATGACATGT
4921 TTGGGTCTTTTATACATGAAAATTTGTTTGACAATAATCTCACAAAACATATTTTACATC
4981 TGAACAAAATGCCTTTTTGTTTACCGTAGCGTATACATTTGTTTTGGGATTTTTGTGTGT
5041 TTGTTGGGAATTTTGTTTTTAGCCAGGTCAGTATTGATGAGGCTGATCATTTGGCTCTTT
5101 TTTTCCTTCCAGAAGAGTTGCATCAACAAAGTTAATTGTATTTATGTATGTAAATAGATT
5161 TTAAGCTTCATTATAAAATATTGTTAATGCCTATAACTTTTTTTCAATTTTTTTGTGTGT
5221 GTTTCTAAGGACTTTTTCTTAGGTTTGCTAAATACTGTAGGGAAAAAAATGCTTCTTTCT
5281 ACTTTGTTTATTTTAGACTTTAAAATGAGCTACTTCTTATTCACTTTTGTAAACAGCTAA
5341 TAGCATGGTTCCAATTTTTTTTAAGTTCACTTTTTTTGTTCTAGGGGAAATGAATGTGCA
5401 AAAAAAGAAAAAGAACTGTTGGTTATTTGTGTTATTCTGGATGTATAAAAATCAATGGAA
5461 AAAAATAAACTTTCAAATTGAAATGACGGTATAACACATCTACTGAAAAAGCAACGGGAA
5521 ATGTGGTCCTATTTAAGCCAGCCCCCACCTAGGGTCTATTTGTGTGGCAGTTATTGGGTT
5581 TGGTCACAAAACATCCTGAAAATTCGTGCGTGGGCTTCTTTCTCCCTGGTACAAACGTAT
5641 GGAATGCTTCTTAAAGGGGAACTGTCAAGCTGGTGTCTTCAGCCAGATGACATGAGAGAA
5701 TATCCCAGAACCCTCTCTCCAAGGTGTTTCTAGATAGCACAGGAGAGCAGGCACTGCACT
5761 GTCCACAGTCCACGGTACACAGTCGGGTGGGCCGCCTCCCCTCTCCTGGGAGCATTCGTC
5821 GTGCCCAGCCTGAGCAGGGCAGCTGGACTGCTGCTGTTCAGGAGCCACCAGAGCCTTCCT
5881 CTCTTTGTACCACAGTTTCTTCTGTAAATCCAGTGTTACAATCAGTGTGAATGGCAAATA
5941 AACAGTTTGACAAGTACATACACCATA
KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
ADDITIONAL OLIGOS
start len tm gc% any 3' seq
1 LEFT PRIMER 4412 20 60.01 55.00 2.00 0.00 CATTTCCCTCCCTCTCTTCC
RIGHT PRIMER 4608 20 59.97 50.00 4.00 0.00 AGGCTGACAAACCATGTTCC
PRODUCT SIZE: 197, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
2 LEFT PRIMER 4412 20 60.01 55.00 2.00 0.00 CATTTCCCTCCCTCTCTTCC
RIGHT PRIMER 4607 20 59.97 50.00 4.00 2.00 GGCTGACAAACCATGTTCCT
PRODUCT SIZE: 196, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
3 LEFT PRIMER 2673 20 60.05 55.00 4.00 3.00 GGGTCACTCCAAGGATCAGA
RIGHT PRIMER 2920 20 59.99 55.00 4.00 1.00 AATGTGGCTCCCCTACACAG
PRODUCT SIZE: 248, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
4 LEFT PRIMER 2671 20 60.05 55.00 5.00 2.00 GAGGGTCACTCCAAGGATCA
RIGHT PRIMER 2920 20 59.99 55.00 4.00 1.00 AATGTGGCTCCCCTACACAG
PRODUCT SIZE: 250, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 51689 0 0 0 5578 0 16264 18966 0 9 339 630 9903
Right 51866 0 0 0 5627 0 16606 18698 0 0 323 616 9996
Pair Stats:
considered 3376, unacceptable product size 3355, high end compl 1, ok 20
primer3 release 1.1.0
(primer3_results.cgi 0.4.0 modified for WI)